| Reporter-Quencher Combinations | |||||||
| 3′ Quencher | |||||||
| BHQ®-1 | BHQ®-2 | BBQ®-650 | Eclipse® | Dabcyl | |||
| Abs range (nm) | 480-580 | 550-650 | 550-750 | 390-625 | 380-550 | ||
| Abs max (nm) | 535 | 579 | 650 | 522 | 453 | ||
| 5′ Reporter | Possible combinations | Abs (nm) | Em (nm) | ||||
| LC® CYAN500 | ✓ | ✓ | ✓ | 450 | 500 | ||
| 6-Fam | ✓ | ✓ | ✓ | ✓ | 495 | 520 | |
| Fluo | ✓ | ✓ | ✓ | ✓ | 495 | 520 | |
| FITC | ✓ | ✓ | ✓ | ✓ | 490 | 525 | |
| Tet | ✓ | ✓ | ✓ | ✓ | 521 | 536 | |
| Joe | ✓ | ✓ | ✓ | 522 | 548 | ||
| Yakima Yellow® | ✓ | ✓ | ✓ | 530 | 549 | ||
| Hex | ✓ | ✓ | ✓ | ✓ | ✓ | 535 | 556 |
| Cy3 | ✓ | ✓ | ✓ | 546 | 563 | ||
| Tamra | ✓ | ✓ | ✓ | ✓ | 564 | 579 | |
| Rox | ✓ | ✓ | ✓ | 576 | 601 | ||
| Texas Red® | ✓ | ✓ | ✓ | 586 | 610 | ||
| LC® Red 610 | ✓ | ✓ | ✓ | 590 | 610 | ||
| LC® Red 640 | ✓ | ✓ | 625 | 640 | |||
| Cy5 | ✓ | ✓ | 646 | 662 | |||
| Cy5.5 | ✓ | ✓ | 683 | 705 | |||
| IRDye®700 | ✓ | ✓ | 685 | 705 | |||
| 5′ Quencher | |||||||
| BHQ®-1 | BHQ®-2 | BBQ®-650 | Eclipse® | Dabcyl | |||
| Abs range (nm) | 480-580 | 550-650 | 550-750 | 390-625 | 380-550 | ||
| Abs max (nm) | 535 | 579 | 650 | 522 | 453 | ||
| 3′ Reporter | Possible combinations | Abs (nm) | Em (nm) | ||||
| 6-Fam | ✓ | ✓ | ✓ | ✓ | 495 | 520 | |
| Fluo | ✓ | ✓ | ✓ | ✓ | 495 | 520 | |
| Joe | ✓ | ✓ | ✓ | 522 | 548 | ||
| Hex | ✓ | ✓ | ✓ | 535 | 556 | ||
| Cy3 | ✓ | 546 | 563 | ||||
| Rox | ✓ | ✓ | ✓ | 576 | 601 | ||
| LC® Red 610 | ✓ | ✓ | ✓ | 590 | 610 | ||
| LC® Red 640 | ✓ | ✓ | 625 | 640 | |||
| Cy5 | ✓ | ✓ | 646 | 662 | |||
| Cy5.5 | ✓ | ✓ | 683 | 705 | |||
